Proteins constitute the structural and motor components of the cell, serving as catalyst for most of the biochemical reactions occurring inside the living organisms. Because of their essential roles, significant efforts are being made to functionally characterise proteins in order to validate them as drug/vaccine candidates, develop biologics or for being used as industrial enzymes. Thereby for most of these applications, achieving an optimal level of protein expression and purification is crucial for downstream applications.
Understanding the need, a high throughput protein production and purification unit has been established [(KIIT-TBI Protein facility (KTPF)], in order to give researchers the access to use state-of-the-art technologies for streamlining their experiments. The facility is equipped with the required instruments like PCR, Biosafety cabinets, Incubator-shakers, 10l Fermenter, FPLC systems etc., that a researcher would need for bacterial, yeast and other eukaryotic cell based expression systems; as well as the required facilities to characterize their proteins. Our team at KTPF, lead by technical experts work closely with our incubates and clients to aid their requirements.
We understand that many researchers need a protein production/purification and characterization service with specific skill sets and technological expertise that they do not possess or wish to establish. The facility also focuses on providing services and technical guidance at all steps starting from cloning to recombinant protein expression and characterization as shown in the organogram. Based on the requirement the user can avail any of these services as below.
However the users will need to provide a minimal charges for availing these services, which will be used towards paying manpower and buying consumables.
|R&D labs||Private Industries|
|RP-HPLC||INR 1200/Run||INR 1600/run|
|FPLC-SEC (without molecular weight standards)||INR 1200/sample||INR 1600/sample|
|FPLC-SEC (with molecular weight standards)||INR 2700/sample||INR 3600/sample|
|Calculate Extinction coefficient||INR 1350/sample||INR 1800/sample|
|Protein Estimation (Bradford/BCA)||INR 750 (1-10 samples)||INR 1000 (1-10 samples)|
|CD Spectroscopy||INR 450/sample||INR 600/sample|
|DLS||INR 450/sample||INR 600/sample|
|Intact Mass||INR 2700/sample||INR 3600/sample|
|Protein Identification by Peptide Mass Fingerprinting||INR 3000/sample||INR 4000/sample|
|Trypsin digestion and peptide extraction from gel spots||INR 1500/spot||INR 2000/spot|
|In solution trypsin digestion (low complexity samples, i.e. purified protein)||INR 1500/sample||INR 2000/ sample|
|In solution digestion (high complexity, i.e. organelle extract or whole cell)||INR 2100/sample||INR 2800/sample|
# Pricing for other Services like Cloning, expression/purification or any other type of analysis will be charged extra as per the users requirement.